Single Cell / FACS / FFPE
SINGLE CELL SEQ
SINGLE CELL SEQ
SMARTer and 10X Genomics approaches for single cell sequencing
FACS CELLS
FACS
Sequencing of cells isolated using FAC sorting- including low cell count preparations
FIXED TISSUE
FIXED TISSUE
Extracting and sequencing samples from Formalin Fixed Parafin Embedded (FFPE) Samples
RNA-sequencing on FACS sorted cells allows for characterization of heterogeneous cell function in a tissue sample, which provide the possibility for precision study.
The omiics team has a wealth of experience in working with FACS sorted cells.
We have different kinds NGS solutions for the samples with different input cell numbers, no matter how low the number of cells!
To dissect the inherent functionalities of tissue heterogenity, the use of single cell sequencing is rising.
omiics is experienced with the 10X Genomics technology and offer bioinformatic analysis of resulting data.
Furthermore, we offer single nuclei sequencing. Single cell sequencing requires fresh material, as the cell membrane integrity is disturbed by freezing. Hence, too much noice is generated due to transcripts from ruptured cells. An alternative for frozen material is single nuclei.
Compared to fresh frozen tissue, the RNA in formalin-fixed, paraffin-embedded (FFPE) tissue is highly degraded and chemically modified. But the abundance, availability and rich clinical data make FFPE samples very interesting to investigate.
The omiics team can provide both a NGS solution and nanoString panels designed for FFPE samples.
What to expect in your report from omiics
NGS service provider with a focus on customer needs. We deliver flexible solutions to your specific demands.
Contact us for a non-binding discussion about your next project.
