Once sequencing libraries have passed the final step of quality control, sequencing will be performed Multiple competitive technologies are available, each having their specific advantages
Technology
Stranded sequencing
Next Generation Sequencing (Illumina/MGI-DNB) or
High-Throughput Next Generation detection (NanoString) or
Third Generation Sequencing (Oxford Nanopore Technology)
Non-stranded sequencing is available too, however, gives you less valuable data and while historical cheaper, the current prices are identical.
The Illumina sequencing-by-synthesis technology is currently the most extensively used NGS technology worldwide. The current options covers several diffent sequencer models, differing in capacity, speed and price:
MiSeq, HiSeq, NextSeq and NovaSeq
Originally developed by MGI, the DNBseq sequencing technology was recently aqquired by the BGI corporation. DNB employ DNA NanoBalls, linear Rolling Circle Replication and cPAS technology. Illumina SBS libraries can be converted to be compatible with DNB technology.
Oxford Nanopore technologies have created a series of biosensors measuring changes in electic current. The smallest member for the family, the MinIon, has a strong potential for suitcase-solution for performing on-site sequencing.
The nanoString nCounter uses a probe-based enzyme-free detection technology. At omiics, we can help you using nanoString for
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NGS service provider with a focus on customer needs. We deliver flexible solutions to your specific demands.
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