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SEQUENCING

Once sequencing libraries have passed the final step of quality control, sequencing will be performed Multiple competitive technologies are available, each having their specific advantages

Technology 





Stranded sequencing

Next Generation Sequencing (Illumina/MGI-DNB) or 

High-Throughput Next Generation detection (NanoString) or

Third Generation Sequencing (Oxford Nanopore Technology)


Non-stranded sequencing is available too, however, gives you less valuable data and while historical cheaper, the current prices are identical.

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sequencing

Most popular

NEXT GENERATION SEQUENCING - SBS


The Illumina sequencing-by-synthesis technology is currently the most extensively used NGS technology worldwide. The current options covers several diffent sequencer models, differing in capacity, speed and price: 

MiSeq, HiSeq, NextSeq and NovaSeq  

NEXT GENERATION SEQUENCING - DNB


Originally developed by MGI, the DNBseq sequencing technology was recently aqquired by the BGI corporation. DNB employ DNA NanoBalls, linear Rolling Circle Replication and cPAS technology. Illumina SBS libraries can be converted to be compatible with DNB technology.  

New in omiics

THIRD GENERATION SEQUENCING


Oxford Nanopore technologies have created a series of biosensors measuring changes in electic current. The smallest member for the family, the MinIon, has a strong potential for suitcase-solution for performing on-site sequencing.  

Long-read technology

NEXT GENERATION DETECTION


The nanoString nCounter uses a probe-based enzyme-free detection technology. At omiics, we can help you using nanoString for 


  • Alternative Detection 
  • NGS Validation


Read more about our expert sample preparation

Get an impression of the types of custom analyses we can perform for your project

See examples of our publications and visualizations