RNA DISTRIBUTION
When doing small RNA sequencing, miRNAs are often the main RNA-type of interest (read more about mRNA target analysis here). Often other RNA-types hold valuable information that should not be overlooked.
We detect the distribution of RNA species by quntifying the number of sequencing reads that map to RNA types such as miRNA, tRNA, snoRNA, snRNA, vaultRNA, mRNA and rRNA.
tRNA molecules are cleaved into smaller fragments, which have been shown to be involved in multiple disease states and in response to stress conditions (read more about tRNA profiling here). However, snoRNA, snRNA, vaultRNA have likewise been shown to be cleaved but the function of these clevage products are less studied.
The amount of reads mapping to ribosomal RNA (rRNA) can often be use as a proxy for RNA degradation, since the size of rRNA far exceedes the size limits of RNAs sequenced in small RNA-seq, while degraded RNA samples will include large amounts of cleaved rRNA sequences in the target size range.
You will recieve visualizations of the distribution of reads for each sample. For example, a stacked barplot where samples are sorted by miRNA
percentage.
NGS service provider with a focus on customer needs. We deliver flexible solutions to your specific demands.
Contact us for a non-binding discussion about your next project.
