Circlar RNA species (CircRNAs) are formed via by back-splice events, producing a characteristic backsplice-junction (bsj). CircRNAs are normally produced from genes also producing a linear mRNA or lncRNA via normal linear splicing. Quantification of circRNAs occurs by detection of the bsj.
While the expression level of circRNAs are much lower than the parental transcript, NGS makes it possible to quantify the expression level of circRNA.
The existence of circular RNAs (circRNAs) has been recognized since the 1980s, but the specific functionalities were only first reported in 2013 (authored by omiics senior adivsor Jørgen Kjems). Since then, there has been massive interest in the potential of this novel class of regulators.
OUTPUT FROM circRNA DETECTIONANALYSIS
Just like in standard differential expression, Principal Component Analysis (PCA) Plot is used to give you a quick visual overview of the general difference between your samples in CircRNA expression.
The Volcano plot is a visual representation of all CircRNAs, correlating the change in expression level between the samples. Each dot denotes a specific CircRNA. Black dots represent non-significant changes, while red dots display significant values.
LIST OF circRNA EXPRESSION
In addition to comparisons between groups, you will get values for ratios between circRNA and the parental host transcript within each sample.