Alternative splicing analysis allows you to identify or compare novel RNA variants from the same gene.
Once a full-length RNA strand is formed, parts of it may have been removed via alternative splicing. In this way, one gene can produce multiple different RNAs with similar, yet non-identical functions.
NGS gives you the full scope of RNA variants produced on a genomewide scale. By applying bioinformatics, you can discover novel splice variants, detect significant changes in alternative splicing, and find altered distribution of variants (a.k.a. isoforms).
OUTPUT FROM ALTERNATIVE SPLICING ANALYSIS
Alternative splicing events
The following alternative splicing events are examined:
Skipped exon (SE)
Mutually exclusive exons (MXE)
Alternative 3' splice site (A3SS)
Alternative 5' splice site (A5SS)
Retained intron (RI)
You will get a table of the total number of these alternative splicing events detailing how many are significantly differentially observed between groups
The most pronounced changes will be visualized using sashimi plots for visual comparison and use in scientific publications.
Significantly regulated events will be examined for RNA binding protein motifs, producing visual outputs also suitable for scientific publication. Knowledge of RNA binding protein motifs associated with alternative splicing can help uncover pathological mechanism in disease and drive hypothesis creation.