Total RNA-seq, transcriptome analysis, mRNA-seq, Long non-coding RNA-seq, RNA-seq for quantification & 3’end sequencing
Characterizing transcriptome dynamics by sequencing
While the genome in an organism is considered to be mostly static over time, changes associated with development, aging and disease is reflected in the transcriptome. Comparative studies applying RNA-seq enables investigation of changes in expression level, isoform usage and regulatory events as circularization.
While each project is unique and consequently the optimal solution varies according to the settings, some elements will be fairly consistent across most experiements.
A complete omiics standard project for small RNA sequencing include:
The project starts with a meeting with a NGS-scientist, where your research
and material is discussed and the optimal startegy is designed.