MicroRNA (miRNA) is known to as a vital control mechanism. Each miRNA can control hundreds of messenger RNAs (mRNA), and a mRNA is most often under the control of several miRNAs, resulting in a finely balanced, intricate network of control.
Gaining insight into small RNA expression signature in cells, tissue and biofluid of interest can have great impact on biomarker discovery, detection of treatment targets and insight into mechanisms involved.
While each project is unique and consequently the optimal solution varies according to the settings, some elements will be fairly consistent across most experiements.
A complete omiics standard project for small RNA sequencing include:
The project starts with a meeting with a NGS-scientist, where your research
and material is discussed and the optimal startegy is designed.