To prepare your material for deep-sequencing, three essential steps are required in the laboratory. At omiics, we have the facilities and experience to conduct all three for you.
Today there is a wide selection of library preparation kits available and which one is the best choice depends on both the input quality and quantity, as well as, the type of end-analysis needed.
Make it easy for yourself - Let omiics choose the optimal reagents for your setup.
Deplete the many or enrich the few?
Ribosomal RNA (rRNA) comprises a significant proportion (~90% or more) of all the RNA in total RNA samples. While the rRNA will not directly cause harm to the result, removing rRNA or enrichment of mRNA prior to generating libraries provides benefits by lowering sequencing costs and improving mapping statistics.
We highly recommend using rRNA removal as this provides data on both coding and noncoding RNAs. While historically polyA enrichment has been cheaper, the current prices are comparable,
- so why settle for less infomation?
Unique molecular identifyer (UMI)
A speciality of omiics is sequencing ultra-low input samples down to as low as 250pg RNA.
At ultra-low RNA concentration, the sample becomes more sensitive to PCR-associated bias. By including an additisonal molecular tag during library preparation, the unique molecular identifyer, UMI, enables you to get high-resolution reads, to facilitate accurate detection of true variants.