LABORATORY FACILITIES




NEXT GENERATION SEQUENCING

LABORATORY PREPARATION OF MATERIAL FOR SEQUENCING 


To prepare you material for deep-sequencing, three essential steps are required in the laboratory. At omiics, we have the facilities and experience to conduct all three for you.  

RNA extraction

Isolation of RNA from cells, tissues or biofluids


Read more below

Library preparation

Prepare RNA for sequencing


Read more below

Quality Control

Test the library quality before sequencing 


Read more below


OTHER SERVICES

OTHER LABORATORY SERVICES 


At the omiics laboratory unit, we perform additional services as well, and the selection keeps growing.   

Nanostring

Validation of NGS data 


Read more below

Special requests

Other methods and equipment 


Read more below

RNA EXTRACTION

To perform sequencing of your sample, we initially isolate RNA. The optimal procedure is depends on whether you  are interested in:


  • Total long RNA (mRNA, lncRNA, circRNA) or
  • Small RNAs (miRNA, tRNA)  

LIBRARY PREPARATION

SAMPLE MATERIAL SPECIFICATION


TYPE   

omiics has versatile experience with sample material from tissue, cells, biofluids and powders. Examples include muscle and brain tissue, mammalian cells, exosomes, blood, CSF, bile, saliva and food powders. 



QUANTITY       

We specialize in low-input samples and have successfully sequenced samples using input as low as 250pg RNA. For standard setups we recommend input of 500ng (total RNA) to 1ug (small RNA) for sequencing.  



QUALITY     

RNA is a highly unstable molecule. It is crucial to handle samples in RNase-free environment and keep samples below 4C at all times. As a standard, RNA should have a RIN value above 7 for optimal sequencing. Partially degraded RNA samples can also be sequenced.

TOTAL LONG RNA


REMOVING rRNA 

Ribosomal RNA (rRNA) comprises a significant proportion (~90% or more) of all the RNA in total RNA samples. Depleting rRNA from total RNA prior to generating libraries provides benefits by lowering sequencing costs and improving mapping statistics.

TOTAL LONG RNA & SMALL RNA

Today there is a wide selection of library preparation kits available and which one is the best choice depends on both the input quality and quantity, as well as, the type of end-analysis needed. Let omiics choose the optimal reagents for your setup.  

QUALITY CONTROL

QUALITY CONTROL

Before we make the final decision to sequence, we check the quality of the finished libraries using the Bioanalyzer technology.


CONCENTRATION

The concentration is determined with high accuracy using a specialized qPCR protocol 

OTHER SERVICES - NANOSTRING

NANOSTRING


The Nanostring technology constitutes a valuable approach to screening the expression level of selected candidates. It can serve as a simplified alternative to NGS, or it can be used to validate the expression of interesting candidates identified by NGS to improve the impact of the findings. Nanostring is often viewed as a time-saving alternative to qPCR validation.

OTHER SERVICES - SPECIAL REQUESTS

ADDITIONAL METHODS AND EQUIPMENT


Did you not find what you were looking for? - Please feel free to contact us. 


At omiics we have an extensive network of collaborators, both in academic and company settings. If we don't have access, we may know someone who does.

CONTACT US

Åbogade 15, 8200 Århus N, Denmark

  Tel: (+45) 28727107

PROFILE

RNA NGS service provider with a focus on customer needs. We provide flexible knowledge based answers to your RNA quantification needs.

Contact us for a non-binding discussion about your next project.

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