COUNSELING AND DESIGN
Tell us about Your research
and we will help find the optimal NGS solution for you
DESIGNING AN OPTIMAL NGS PIPELINE
The first step for us is to learn about your research. Based on your material and scientific question, we tailor the approach to match your needs.
We do this at an initial meeting, phone call or via emails.
Things to consider before you start
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Choosing between long and small RNA sequencing
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WHAT IS OPTIMAL AND WITH WHAT IS POSSIBLE
Based on your material and scientific question, together we discuss different options for the experimental design, e.g. the appropriate number of samples/replicates, optimal storage and handling, and choice of sequencing technique.
We specialize in sequencing of difficult samples. Often samples with low RNA content are the most precious, so why not let us unfold the potential of your precious samples by performing miRNA or total RNA sequencing? Low input samples include plasma, exosome purification, cerebrospinal fluid (CSF), laser microdissected tissue and more.
It is important to consider what sources of bias your experiment could have. These could be sex, age, the time since samples were taken, sample quality and much more.
Groups to be compared by NGS must be as similar as possible in the potential bias-causing attributes to ensure that significant hits are real and not caused by bias.
After an initial discussion, we will make a plan for library preparation and sequencing. In general, the amount of your sample and quality of the RNA are central factors for which preparation method is the optimal choice. The final libraries produced are quality controlled before moving on to sequencing.
Our best selling product is the complete quantification of long RNAs in customer samples. This includes mRNA and long noncoding RNA species. Our standard total RNA-seq package includes differential expression analysis and publication grade plotting of results. We also specialize in analysis of circular (circRNA) from total RNA data.
Total RNA sequencing can be used for analysis of: mRNA, lncRNA, circRNA
Small RNAs provide vital cellular control mechanisms. In disease states and during developmental processes, the network of miRNAs and other small RNAs is often affected. Gaining insight into the miRNA expression signature in cells/tissue/fluid of interest can have great impact on biomarker discovery and insight into mechanisms involved and much more.
Small RNA sequencing can be used for analysis of: miRNA, tRNA, siRNA, piRNA, snoRNA, vaultRNA and more
At the initial consultation, we also discuss what bioinformatic analysis would be relevant for your question. In the case of standard analysis, we have a defined package of figures and tables to give you both easy overview and the catalogue to zoom in on details on single transcripts.
Åbogade 15, 8200 Århus N, Denmark
Tel: (+45) 28727107
RNA NGS service provider with a focus on customer needs. We provide flexible knowledge based answers to your RNA quantification needs.
Contact us for a non-binding discussion about your next project.
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