LOW INPUT SAMPLES

At omiics, we specialize in low input samples for RNA NGS.


Because of our impeccable laboratory work and expert customized bioinformatics, you can fully trust the results and focus on the implications of the data.


SINGLE CELL SEQ


SINGLE CELL SEQ


SMARTer and 10X Genomics approaches for single cell sequencing 


FACS CELLS

FACS


Sequencing of cells isolated using FAC sorting- including low cell count preparations


FIXED TISSUE


FIXED TISSUE 


Extracting and sequencing samples from Formalin Fixed Parafin Embedded (FFPE) Samples


BIOFLUIDS


BIOFLUIDS


Cell-free samples. Including plasma,  cerebrospinal fluid (CSF), salvia, urine, bile, and more!


EXOSOMES/EV

EXOSOMES/EV


 Special example of cell-free samples:  isolating & sequencing exosomes/extracellular vesicles (EV)

RNA-sequencing on FACS sorted cells allows for characterization of heterogeneous cell function in a tissue sample, which provide the possibility for precision study.


The omiics team has a wealth of experience in working with FACS sorted cells.


We have different kinds NGS solutions for the samples with different input cell numbers, no matter how low the number of cells!

Fluorescence-activated cell sorting (FACS) Samples

Check out our paper on

SMART-Seq in FACS 

Single cell sequencing

SMART-Seq / 10X Genomics


To dissect the inherent functionalities of tissue heterogenety, the use of single cell sequencing is rising. 

SMART-seq Single Cell


For an end-to-end single cell sequencing solution, we offer SMART-Seq


One methodolody involve the use of SMARTer Stranded Total RNA kit, which enables combines strandeness and effective removal of ribosomal cDNA on a low number of cell, even single cell, such as FACS cells.


Previous project, were we used the technology: 



10 X Genomics


omiics is experienced with the 10X Genomics technology and offer bioinformatic analysis of resulting data.


Furthermore, we can facilitate access to the nessesary equipment for academic research collaborations, however, currently we do not have licence for commercial projects. 


Biofluids


Cell-free biofluids have enormous diagnostic and clincal potential. The quantity of RNAs in the fluids can be exceptionally low and expert sample preparation is recommended.


omiics is experienced with plasma, cerebrospinal fluid (CSF), salvia,  bile, etc.

We have even sucessfully identified tRNAs in human embryo culture media!

Fixed Tissue 

Compared to fresh frozen tissue, the RNA in formalin-fixed, paraffin-embedded (FFPE) tissue is highly degraded and chemically modified. But the abundance, availability and rich clinical data make FFPE samples very interesting to investigate.


The omiics team can provide both a NGS solution and nanoString panels designed for FFPE samples.


Exosome / Extracellular Vesicles

ISOLATION AND SEQUENCING


Exosome/ EV text


The omiics team has extensive expertise with working with exosomes/EVs. Here is our published protocol, together with comments and tips for isolating EVs from cell media and small RNA sequencing of EVs. We are very happy to discuss your project and share our experience with you