The nanoString nCounter uses a probe-based enzyme-free detection technology. Nanostring does not rely on enzymatic activity for reverse transcription of the RNA but rather on a pre-formatted panel of probes.
At omiics, we can help you design your own probes and nanostring provides reporter and tagset. This makes nanoString more flexible.
nanoString Technology may be an ideal solution if you:
are working with damaged and degraded RNA samples such as FFPE
have too many targets for traditional qPCR
only want to detect absolute expression of RNA molecules and do not want to take any risk of enzyme bias
NGS Validation with nanoString
Validation of multiple candidate mRNAs/lncRNAs/circRNAs in a large cohort of samples can be done quickly and efficiently using NanoString. nCounter® PlexSet™.
Reagents enable validation of up to 96 mRNA/circRNA targets (12, 24, 48 or 96 targets) across 96 samples in one run. It can be done directly on cell lysate without RNA purification.
The nCounter® PlexSet™ may be an ideal solution if you:
do not have time to do RNA purification, reverse transcription and qPCR on many samples
want to get the data quickly
have more than 10 targets to valiate
want to do robust validation of NGS data using enzyme free technique